LOCUS       sequence-82679-e         315 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus
            is fused in frame with the luciferase in the herpes simplex virus 
            type-1 amplicon vector, provides prolonged transgene expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Sia KC, Chong WK, Ho IA, Yulyana Y, Endaya B, Huynh H, Lam PY
  TITLE     Hybrid herpes simplex virus/Epstein-Barr virus amplicon viral 
            vectors confer enhanced transgene expression in primary human tumors
            and human bone marrow-derived mesenchymal stem cells.
  JOURNAL   J Gene Med. 2010 Oct;12(10):848-58. doi: 10.1002/jgm.1506.
  PUBMED    20963807
REFERENCE   2  (bases 1 to 315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jgm"; 
            journalName: "J Gene Med"; date: "2010-10"; volume: "12"; issue: 
            "10"; pages: "848-58"
COMMENT     Sequence Label: pHCAG-L2EOP
FEATURES             Location/Qualifiers
     source          1..315
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..82
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(256..278)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccacctcgat atgtgcatct gtaaaagcaa ttgttccagg aaccagggcg tatctcttca
       61 tagccttatg cagttgctct ccagcggttc catcttccag cggatagaat ggcgccgggc
      121 ctttctttat gtttttggcg tcttccatgg tggctcgagc ggccgccact gtgctggata
      181 tctgcagaat tccaccacac tggactagtg gatccgagct cggtaccaag cttaagttta
      241 aacgctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc gtcccccccg
      301 aaggcagccg tctgc
//
