LOCUS       sequence-8268-ex        2779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Zarse K, Schulz TJ, Birringer M, Ristow M
  TITLE     Impaired respiration is positively correlated with decreased life 
            span in Caenorhabditis elegans models of Friedreich Ataxia.
  JOURNAL   FASEB J. 2007 Apr . 21(4):1271-5.
  PUBMED    17215485
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2007 Apr . 21(4):1271-5."
COMMENT     Sequence Label: frh-1-C
FEATURES             Location/Qualifiers
     source          1..2779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(170..186)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(171..187)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(212..230)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(240..257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(240..256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(249..271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      398..853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(485..504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     695..716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        879..983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(984..1052,1053..1844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1202..1221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2015..2603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2504..2523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2757..2774
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatgggga
       61 acgtatgtta ttaacaaaca atcaccaaat aagcaaattt ggttgtccag tccgatgtct
      121 ggtccgaaaa gatatgattt agaagaggag ggaaacccat caagcttatc gataccgtcg
      181 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
      241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      361 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
      421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
      841 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1681 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2161 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2761 agtcagtgag cgaggaagc
//
