LOCUS       sequence-82682-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal HA tagged hM4Dnrxn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: CMV:: HA-hM4Dnrxn
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 caccatgtac ccatacgatg ttccagatta cgctatggcc aacttcacac ctgtcaatgg
      181 cagctcgggc aatcagtccg tgcgcctggt cacgtcatca tcccacaatc gctatgagac
      241 ggtggaaatg gtcttcattg ccacagtgac aggctccctg agcctggtga ctgtcgtggg
      301 caacatcctg gtgatgctgt ccatcaaggt caacaggcag ctgcagacag tcaacaacta
      361 cttcctcttc agcctggcgt gtgctgatct catcataggc gccttctcca tgaacctcta
      421 caccgtgtac atcatcaagg gctactggcc cctgggcgcc gtggtctgcg acctgtggct
      481 ggccctggac tgcgtggtga gcaacgcctc cgtcatgaac cttctcatca tcagctttga
      541 ccgctacttc tgcgtcacca agcctctcac ctaccctgcc cggcgcacca ccaagatggc
      601 aggcctcatg attgctgctg cctgggtact gtccttcgtg ctctgggcgc ctgccatctt
      661 gttctggcag tttgtggtgg gtaagcggac ggtgcccgac aaccagtgct tcatccagtt
      721 cctgtccaac ccagcagtga cctttggcac agccattgct ggcttctacc tgcctgtggt
      781 catcatgacg gtgctgtaca tccacatctc cctggccagt cgcagccgag tccacaagca
      841 ccggcccgag ggcccgaagg agaagaaagc caagacgctg gcct
//
