LOCUS       sequence-82684-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of Sirius, a pH-insensitive and photostable 
            ultramarine fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa
            K, Nagai T
  TITLE     An ultramarine fluorescent protein with increased photostability and
            pH insensitivity.
  JOURNAL   Nat Methods. 2009 May;6(5):351-3. doi: 10.1038/nmeth.1317. Epub 2009
            Apr 6.
  PUBMED    19349978
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1317"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "351-3"
COMMENT     Sequence Label: Sirius/pRSETB
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             473..724
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK"
ORIGIN
        1 ggatccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagctcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgcaattcgg cgtgctgtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca acgggataag ctcaaacgta tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcgtcc agtccaagct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagtccg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      721 caagtaagaa ttc
//
