LOCUS       sequence-82691-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - 
            PHLS gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Formighieri C, Melis A
  TITLE     Regulation of beta-phellandrene synthase gene expression, 
            recombinant protein accumulation, and monoterpene hydrocarbons 
            production in Synechocystis transformants.
  JOURNAL   Planta. 2014 Aug;240(2):309-24. doi: 10.1007/s00425-014-2080-8. Epub
            2014 May 20.
  PUBMED    24838596
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00425-014-2080-8"; journalName: "Planta"; date: "2014-08";
            volume: "240"; issue: "2"; pages: "309-24"
COMMENT     Sequence Label: psbA2-Ptrc-PHLS (d)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(416..421,422..438,439..445)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    454..470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggatatttgc tgggggttaa tgaaacattg tggcggaacc cagggacaat gtgaccaaaa
       61 aattcaggga tatcaataag tattaggtat atggatcata attgtatgcc cgactattgc
      121 ttaaactgac tgaccactga ccttaagagt aatggcgtgc aaggcccagt gataaatttc
      181 attatttttc attatttcat ctccattgtc cctgaaaatc agttgtgtcg cccctctaca
      241 cagcccagaa ctatggtaaa ggcgcacgaa aaaccgccag gtaaactctt ctcaaccccc
      301 aaaacgccct ctgtttaccc atggaaaaaa cgacaattac aagaaagtaa aacttatgtc
      361 atctataagc ttcgtgtata ttaacttcct gttacaaaga ttctgaaatg agctgttgac
      421 aattaatcat ccggctcgta taatgtgtgg aaattgtgag cggataacaa ttaggaggta
      481 aacatatgtg tagtttgcaa gtttctgatc ctattcctac cggacgccgt tccggtggtt
      541 atcccccggc cttatgggat ttcgatacta ttcaatccct gaataccgaa tataagggcg
      601 aacgtcacat gcgtcgggaa gaagacttaa ttggtcaagt tcgggaaatg ttggtgcacg
      661 aagtagaaga tcccactccc cagttggaat tcattgacga tctgcataaa ttgggcattt
      721 cctgccattt tgaaaacgag attctgcaaa ttctcaaatc catttatctc aaccaaaact
      781 ataaacggga cctctattct accagtttag ccttccgtct cttgcgtcaa tacgggttta
      841 tcttgccgca ggaagttttt gactgcttt
//
