LOCUS       sequence-82704-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, 
            Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Blast V5-LUC (w528-1)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(112..153)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(165..189)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(244..754)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(307..325)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa cagtaccgga
       61 atgccaagct ttttgcaaaa gcctagggac tgaattggtt cccatggatc ccgtagaatc
      121 gagaccgagg agagggttag ggataggctt accttccatg gtggagcctg cttttttgta
      181 caaacttgtt gatatctgca gaattccacc acactggact agtcgacgaa ccggtggatc
      241 cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc
      301 cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc
      361 acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca
      421 gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc
      481 gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa
      541 ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag
      601 ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg
      661 gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc
      721 agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttcgatatca agcttctcga
      781 ggctagtctc gtgatcgata
//
