LOCUS       sequence-82705-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Kss1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan 
            DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A,
            Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, 
            Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein
            MB, Snyder M, Turk BE
  TITLE     Deciphering protein kinase specificity through large-scale analysis 
            of yeast phosphorylation site motifs.
  JOURNAL   Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482.
  PUBMED    20159853
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2010-02-16"; volume: "3(109):ra12. doi"; pages: " 
            10.1126/scisignal.2000482"
COMMENT     Sequence Label: pET28-Kss1
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..93
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             97..129
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
       61 catcacagca gcggcctggt gccgcgcggc agccatatgg ctagcatgac tggtggacag
      121 caaatgggtc gcggatccaa gatggctaga accataactt ttgatatccc ttcccaatat
      181 aaactcgtag atttaatagg tgagggagcg tacggaacag tatgttcagc aattcataag
      241 ccttccggca taaaggtagc tatcaagaaa atacaaccgt ttagcaaaaa attgtttgtt
      301 acaagaacta tacgtgagat caagctttta cggtatttcc atgaacacga aaacataata
      361 agtatattgg ataaagtaag gccagtatcc atagacaaac taaacgctgt ttatttagtc
      421 gaagagttga tggaaaccga tttacaaaaa gtaattaata atcagaatag cgggttttcc
      481 actttaagtg atgaccatgt tcaatacttt acataccaaa tcctcagagc cttaaagtct
      541 attcacagtg cacaagttat ccatagagac ataaagccat caaacctgtt actaaattcc
      601 aattgtgatc tcaaagtctg cgattttgga ctagcgaggt gtttagctag cagtagcgat
      661 tcaagagaaa cattggtagg attcatgacg gagtacgtcg caacgcgatg gtacagggca
      721 cccgagataa tgctaacttt tcaagagtac acaactgcga tggatatatg gtcatgcgga
      781 tgcattttgg ctgaaatggt ctccgggaag cctttgttcc caggcagaga ctatcatcat
      841 caattatggc taattctaga agtcttgg
//
