LOCUS       sequence-82728-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD
  TITLE     NEMO is a sensor of Lys 63-linked polyubiquitination and functions 
            in NF-kappaB activation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):398-406.
  PUBMED    16547522
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):398-406."
COMMENT     Sequence Label: pCMVTAG-NEMO
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..65
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact
       61 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat
      121 gacgacgata agagcccggg cggatccccc gggctgcagg aattcaatag gcacctctgg
      181 aagagccaac tgtgtgagat ggtgcagccc agtggtggcc cggcagcaga tcaggacgta
      241 ctgggcgaag agtctcctct ggggaagcca gccatgctgc acctgccttc agaacagggc
      301 gctcctgaga ccctccagcg ctgcctggag gagaatcaag agctccgaga tgccatccgg
      361 cagagcaacc agattctgcg ggagcgctgc gaggagcttc tgcatttcca agccagccag
      421 agggaggaga aggagttcct catgtgcaag ttccaggagg ccaggaaact ggtggagaga
      481 ctcggcctgg agaagctcga tctgaagagg cagaaggagc aggctctgcg ggaggtggag
      541 cacctgaaga gatgccagca gcagatggct gaggacaagg cctctgtgaa agcccaggtg
      601 acgtccttgc tcggggagct gcaggagagc cagagtcgct tggaggctgc cactaaggaa
      661 tgccaggctc tggagggtcg ggcccgggcg gccagcgagc aggcgcggca gctggagagt
      721 gagcgcgagg cgctgcagca gcagcacagc gtgcaggtgg accagctgcg catgcagggc
      781 cagagcgtgg aggccgcgct ccgcatggag cgccaggccg cctcggagga gaagaggaag
      841 ctggcccagt tgcaggtggc ctatcaccag ctcttccaag aatacgacaa ccacatcaag
//
