LOCUS       sequence-82732-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter for normal human GPR56 exon 1m promoter activity. Human 
            GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted
            into pGL3E..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang 
            PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, 
            Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh 
            CA
  TITLE     Evolutionarily dynamic alternative splicing of GPR56 regulates 
            regional cerebral cortical patterning.
  JOURNAL   Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
  PUBMED    24531968
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-14- 14"; volume: "343"; 
            issue: "6172"; pages: "764-8"
COMMENT     Sequence Label: pGL3E-hGPR56 e1m promoter normal
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      417..426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(489..507)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc ccgggctcga gatctgngat
       61 ctaagtaagc ttccccataa atcgctgtcc taacccctgc cctccctcct gccagctccc
      121 tgtctggcct gggcagcgtc tgagttgagg acttgggaac aggacaagtt acggagccac
      181 gttgctttgc tgggtctgag ccggggtgtg acgtagtccc tgcagctgcc aacggttgcc
      241 agggcaacgg ttgccagggg ctgctgtcac ctgcgcccct tctcccgcgc tggcggctgg
      301 ggcttctcag cctctattcc ctggctgtcc cctttgtttg aagctccagt gagggagcag
      361 tggctggggt ggcccagctt caaagtcaag cttggcattc cggtactgtt ggtaaagcca
      421 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      481 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      541 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      601 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      661 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      721 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      781 tttcgcagcc taccgtggtg ttcgtttcca aa
//
