LOCUS       sequence-82742-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pre-2Ato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-pre-mGRASP-2A-dTomato
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..125
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gtcttttgtt gcttgctgca cttctcccct tcgctcttcc cgcttcagat tggaagacag
       61 gagaagtcac cgcttcccgc gaccacatgg tgctgcacga atatgtgaac gctgctggca
      121 tcacaggcgg tggcggatct ggcggcggtg gtagcgtcga ctttcagaaa gctagctcta
      181 ttgtttataa gaaggagggg gaacaggtgg agttcagttt cccattggcc ttcacagtcg
      241 agaagctcac aggatcagga gagttgtggt ggcaggccga aagagcctcc tccagcaaga
      301 gttggatcac ttttgatctg aaaaacaaag aagtgagcgt gaagagagtc acacaggacc
      361 ctaagctgca gatgggcaag aagttgcctt tgcacttgac actcccccaa gccctgcccc
      421 agtatgctgg ctccgggaac ctcaccctcg ccttggaggc taagaccgga aagttgcatc
      481 aggaggtcaa cctcgtggtg atgagggcaa cacagctgca gaagaacctg acctgcgaag
      541 tgtggggtcc cacctcacct aaactgatgc tgtctctgaa gctggagaat aaggaggcta
      601 aagtcagtaa aagagagaag gctgtgtggg tccttaaccc ggaggcaggg atgtggcagt
      661 gtcttcttag cgactctgga caggtgctgc tggagagcaa catcaaggtg cttcctactt
      721 ggtcaacacc agtacagccg atggccctga tcgtactcgg gggagtcgcc ggcctgct
//
