LOCUS       sequence-82743-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter for mutated human GPR56 exon 1m promoter activity. It 
            contains a 15-bp deletion in a conserved noncoding element. The 
            mutated human GPR56 e1m promoter was inserted into pGL3E..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang 
            PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, 
            Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh 
            CA
  TITLE     Evolutionarily dynamic alternative splicing of GPR56 regulates 
            regional cerebral cortical patterning.
  JOURNAL   Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
  PUBMED    24531968
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-02-14- 14"; volume: "343"; 
            issue: "6172"; pages: "764-8"
COMMENT     Sequence Label: pGL3E-hGPR56 e1m promoter perisylvian polymicr...
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      401..410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(473..491)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttctctatcg ataggtaccg agctcttacg cgtgctagcc cgggctcgag atctgcgatc
       61 taagtaagct tccccataaa tcgctgtcct aacccctgcc ctccctcctg ccagctccct
      121 gtctggcctg ggcagcgtct gagttgagga cttgggaaca ggacaagtta cggagccacg
      181 ttgctttgct gggtctgagc cggggtgtga cgtagtccct gcagctgcca acggttgcca
      241 ggggctgctg tcacctgcgc cccttctccc gcgctggcgg ctggggcttc tcagcctcta
      301 ttccctggct gtcccctttg tttgaagctc cagtgaggga gcagtggctg gggtggccca
      361 gcttcaaagt caagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa
      421 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
      481 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc
      541 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga
      601 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc
      661 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc
      721 gaacgacatt ta
//
