LOCUS       sequence-82755-e        8591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of LacZ driven by the human Gfa2 promoter. LacZ can also 
            be replaced with the gene of interest for targeting transgene 
            expression to astrocytes in mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Brenner M, Kisseberth WC, Su Y, Besnard F, Messing A
  TITLE     GFAP promoter directs astrocyte-specific expression in transgenic 
            mice.
  JOURNAL   J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
  PUBMED    8120611
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1994-03"; volume: "14(3 Pt 1)"; pages: "1030-7"
COMMENT     Sequence Label: pGfa2-nLac
FEATURES             Location/Qualifiers
     source          1..8591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2523..2543
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2562..5609
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(6195..6212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6195..6211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6420..6439)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6539..6561
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6599..6617)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6685..6789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6790..6858,6859..7650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7821..8409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8310..8329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8563..8580
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
      241 ggtaccagat ctgagctccc acctccctct ctgtgctggg actcacagag ggagacctca
      301 ggaggcagtc tgtccatcac atgtccaaat gcagagcata ccctgggctg ggcgcagtgg
      361 cgcacaactg taattccagc actttgggag gctgatgtgg aaggatcact tgagcccaga
      421 agttctagac cagcctgggc aacatggcaa gaccctatct ctacaaaaaa agttaaaaaa
      481 tcagccacgt gtggtgacac acacctgtag tcccagctat tcaggaggct gaggtgaggg
      541 gatcacttaa ggctgggagg ttgaggctgc agtgagtcgt ggttgcgcca ctgcactcca
      601 gcctgggcaa cagtgagacc ctgtctcaaa agacaaaaaa aaaaaaaaaa aaaaaaagaa
      661 catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc ctgagctggc
      721 tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac ctggcagcat
      781 tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca ccttggcaca
      841 gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa
      901 tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct gacagcctgg
      961 catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt ggcgccaccg
     1021 gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag gggatgccca
     1081 ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca gaagtccaag
     1141 gacacaaatg ggtgagggga ctgggcaggg ttctgaccct gtgggaccag agtggagggc
     1201 gtagatggac ctgaagtctc cagggacaac agggcccagg tctcaggctc ctagttgggc
     1261 ccagtggctc cagcgtttcc aaacccatcc atccccagag gttcttccca tctctccagg
     1321 ctgatgtgtg ggaactcgag gaaataaatc tccagtggga gacggagggg tggccaggga
     1381 aacggggcgc tgcaggaata aagacgagcc agcacagcca gctcatgtgt aacggctttg
     1441 tggagctgtc aaggcctggt ctctgggaga gaggcacagg gaggccagac aaggaagggg
     1501 tgacctggag ggacagatcc aggggctaaa gtcctgataa ggcaagagag tgccggcccc
     1561 ctcttgccct atcaggacct ccactgccac atagaggcca tgattgaccc ttagacaaag
     1621 ggctggtgtc caatcccagc ccccagcccc agaactccag ggaatgaatg ggcagagagc
     1681 aggaatgtgg gacatctgtg ttcaagggaa ggactccagg agtctgctgg gaatgaggcc
     1741 tagtaggaaa tgaggtggcc cttgagggta cagaacaggt tcattcttcg ccaaattccc
     1801 agcaccttgc aggcacttac agctgagtga gataatgcct gggttatgaa atcaaaaagt
     1861 tggaaagcag gtcagaggtc atctggtaca gcccttcctt cccttttttt tttttttttt
     1921 ttgtgagaca aggtctctct ctgttgccca ggctggagtg gcgcaaacac agctcactgc
     1981 agcctcaacc tactgggctc aagcaatcct ccagcctcag cctcccaaag tgctgggatt
     2041 acaagcatga gccaccccac tcagcccttt ccttcctttt taattgatgc ataataattg
     2101 taagtattca tcatggtcca accaaccctt tcttgaccca ccttcctaga gagagggtcc
     2161 tcttgcttca gcggtcaggg ccccagaccc atggtctggc tccaggtacc acctgcctca
     2221 tgcaggagtt ggcgtgccca ggaagctctg cctctgggca cagtgacctc agtggggtga
     2281 ggggagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
     2341 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
     2401 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
     2461 gcggggatcc tctagagtcg acggatccgg ggaattcccc agtctcagga tccaccatgg
     2521 ggcccaagaa gaaacgcaaa gtggggagca tgggggatcc cgtcgtttta caacgtcgtg
     2581 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2641 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2701 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     2761 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     2821 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc
     2881 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     2941 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
     3001 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
     3061 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca
     3121 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
     3181 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
     3241 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
     3301 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
     3361 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
     3421 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
     3481 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
     3541 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
     3601 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
     3661 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
     3721 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
     3781 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
     3841 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
     3901 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
     3961 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
     4021 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
     4081 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
     4141 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
     4201 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
     4261 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
     4321 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
     4381 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
     4441 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
     4501 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
     4561 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
     4621 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
     4681 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
     4741 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
     4801 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
     4861 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
     4921 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
     4981 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
     5041 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
     5101 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
     5161 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
     5221 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
     5281 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
     5341 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
     5401 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
     5461 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
     5521 gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc
     5581 attaccagtt ggtctggtgt caaaaataat aataaccggg caggggggat ccgcagatcc
     5641 cggccagata ccgatgctgc cgcagcaaaa gcaggagcag atgccgccgt cgcaggcgaa
     5701 gatgtcgcag acggaggagg cgatgctgcc ggcggaggag gcgaagtaag tagagggctg
     5761 ggctgggctg tggggggtgt ggggtgcggg actgggcagt ctgggagtcc ctctcaccac
     5821 ttttcttacc tttctaggat gctgccgtcg ccgccgctca tacaccataa ggtgtaaaaa
     5881 atactagatg cacagaatag caagtccatc aaaactcctg cgtgagaatt ttaccagact
     5941 tcaagagcat ctcgccacat cttgaaaaat gccaccgtcc gatgaaaaac aggagcctgc
     6001 taaggaacaa tgccacctgt caataaatgt tgaaaactca tcccattcct gcctcttggt
     6061 ccttgggctt ggggaggggt gcgcggatgt ggttagggaa catgactggt caaatgggaa
     6121 gggcttcaaa agaattccca atattgacta ccaagccacc tgtacagatc tgcctgcagg
     6181 catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6241 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6301 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     6361 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     6421 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     6481 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     6541 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     6601 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6661 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6721 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6781 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6841 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6901 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6961 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7021 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7081 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7141 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7201 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7261 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7321 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7381 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7441 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7501 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7561 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7621 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7681 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7741 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7801 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7861 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7921 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7981 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8041 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8101 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8161 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8221 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8281 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8341 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8401 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8461 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8521 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8581 gaagcggaag a
//
