LOCUS       sequence-82756-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic vector expressing mRuby2 and GCaMP6m from a single open 
            reading frame. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(97..118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(358..377)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag catggtgagc
       61 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      121 aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta cggcaagctg
      181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      241 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      301 ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt cttcaaggac
      361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      421 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      481 tacaacctgc cggaccaact gactgaagag cagatcgcag aatttaaaga ggctttctcc
      541 ctatttgaca aggacgggga tgggacaata acaaccaagg agctggggac ggtgatgcgg
      601 tctctggggc agaaccccac agaagcagag ctgcaggaca tgatcaatga agtagatgcc
      661 gacggtgacg gcacaatcga cttccctgag ttcctgacaa tgatggcaag aaaagggagc
      721 tacagggaca cggaagaaga aattagagaa gcgttcggtg tgtttgataa ggatggcaat
      781 ggctacatca gtgcagcaga gcttcgccac gtgatgacaa acctt
//
