LOCUS       sequence-82758-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic vector expressing mRuby2 and GCaMP6m from a single open 
            reading frame. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     599..618
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagctctag tgcggccgct tacttcgctg tcatcatttg
       61 tacaaactct tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc
      121 atcaacctct tcatctgtta acttctctcc aaggtttgtc atcacgtggc gaagctctgc
      181 tgcactgatg tagccattgc catccttatc aaacacaccg aacgcttctc taatttcttc
      241 ttccgtgtcc ctgtagctcc cttttcttgc catcattgtc aggaactcag ggaagtcgat
      301 tgtgccgtca ccgtcggcat ctacttcatt gatcatgtcc tgcagctctg cttctgtggg
      361 gttctgcccc agagaccgca tcaccgtccc cagctccttg gttgttattg tcccatcccc
      421 gtccttgtca aatagggaga aagcctcttt aaattctgcg atctgctctt cagtcagttg
      481 gtccggcagg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      541 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      601 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggatgtagc cttcgggcat
      661 ggcggacttg
//
