LOCUS       sequence-82844-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase Reporter with Gal4 DNA binding site upstream of hACTB 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
  TITLE     An AT-hook domain in MeCP2 determines the clinical course of Rett 
            syndrome and related disorders.
  JOURNAL   Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
  PUBMED    23452848
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "984-96"
COMMENT     Sequence Label: pUAS-hActin-luc2
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..186
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      855..864
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttctctggc ctaactggcc ggtacctgag ctcgctagcc tcgaggatat caagatctgg
       61 cctcggcggc caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
      121 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
      181 tccgagcgga gactctagcg agcgccggac atatgactgc ctggccactc catgccctcc
      241 aagagctcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg cagaccctgg
      301 cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg tcccgcggtg
      361 ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg ggccgaaccg
      421 ggcactgccc agcgtggggc gcgggggcca cggcgcgcgc ccccagcccc cgggcccagc
      481 accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct cgctctcgct
      541 cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac tgtgcggcga
      601 agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag ttgcctttta
      661 tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg ccaccaccgc
      721 cgagaccgcg tccgccccgc gagcacagag cctcgccttt gccgatccgc cgcccgtcca
      781 cacccgccgc caggtaagcc cggccagccg accggggcag gcggctcacg aattcgttaa
      841 cagatccgat atccgccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      901 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      961 ccctggtgcc cgg
//
