LOCUS       sequence-82863-e       11369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi 
            to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 
            rescue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11369)
  TITLE     underdominant attB transformation DM plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: {Ud}RpL14.Dm
FEATURES             Location/Qualifiers
     source          1..11369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7585..7679
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7698..7936
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7883..7904
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          8243..8308
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8438..8458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8730..8864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8767..8786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8821..8840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8893..8962
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9181..9199)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9220..9236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9220..9236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9233..9255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9244..9260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9268..9274,9275..9292,9293..9298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9313..9334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9451..9468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9622..10210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9702..9721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10381..11172,11173..11241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11004..11023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11242..11346)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaagctt
     4861 ttagtttcct ggccacttca cgggcatctc gcgcatccgt gggacgcttc aaggtcaagg
     4921 agctctccgg ctccggcttg ggcgccttgt gcgcttgcag tgcctttttc tacgggttaa
     4981 aaacttgtta atataaataa tcacatttgt ggcatgagtt ttcccacctt tttcttgagt
     5041 ttctgatact tggcctgcag catttgctcc tcttcggtta aattgttggg gaagtgtatg
     5101 taaaccatgt tgggaaaatg tctgcttgaa attaaccccg taaaaagtaa cttctacaaa
     5161 agtaattatg ttattccaaa atgtttattt ttactatcga aatctatcga attggcttag
     5221 tgtgactgta acagttgata gcgaaaaata ccacataaga tgtgtatgat ttactgtcac
     5281 attaactatt tctgcacaac actagaccac atgcaactct gtgtttagca acactaacgc
     5341 catgctacca ccgagctgtc aaaaaagttc tttccttctc tttctttccg gttagcgtca
     5401 tggtaagaat cgacgaaggt ttctgcggat cggttgaaaa atgtgaaaat tcagcggtct
     5461 aaatggcccg agatgaatta aatttggcgg ccaagcgaac gggtcagtgc gttgaacgtg
     5521 tgaatagcga aagcagcgta aaagcgttgg aaaaccatgg gatatccaac attcaaatag
     5581 ttcacgtggt ggtcaccaac acaagcaacg cgctggccat gcaacaaccg aaatccaaca
     5641 ttttcaccca tttttaatgg acttctctct gtttgcagcc cttcgagcgc ttcgtgcaaa
     5701 caggccgcat cgccaaagcc tccgccggtc ccctgaaggg gcgcctggtc gccatcgtcg
     5761 atgtcatcga ccagaacagg gtaagtgcat cattccatag tggagtaaca gcaacaaatt
     5821 gccaagtgac gagctcttgc acatgatgat aatttgtctt cttgatagga agtgccaact
     5881 gacacataaa tgaagctagt ttaaccctct gaaagcaagc aagcaaaacc agcatttgtt
     5941 gctgtactcc tcccttcatg gccatacatg gttaaagtga tccaaactaa ttaatggctt
     6001 ttcacttttc aggttctggt agatggtcct ctgactggcg tgccccgtca ggaatacaga
     6061 ttgaacaatc tgcatctgac caagtaccgc atcaagttcc cctacaccgc gcccacgcgc
     6121 atcgtgcgca aggcctggac agagagcgac ctgaaggccc agtggaaggt cagcccctgg
     6181 tccgtcaagg cacagaacat ctgcaaggta tgtacactga gcacgaggag gattcgtagc
     6241 tgtaggaaag tttacctatg tagaggggaa aatgttatac ctcttctctg cctagttgat
     6301 tacctccttg aactgcacat gatgataatt gaaagttctt gataggaatt gccggctgac
     6361 acatatatga agctaattct agtttatctg attacaatca tagctaggcg tcttcataaa
     6421 cattcagaat aaatcaagta ctaatgagaa tttgtcttct tttgcagcgc tcctcgttga
     6481 acgactttga ccgcttcaag ctgcgttatg ccaagcgtca gcgcaacaag ctgctgacca
     6541 tcgccttcaa cacactgaag aagcgcacca aggctgacgg cactccccgc gtcctcaaga
     6601 aggatcgtcg cgagcgtctg cgtgccgaga aggccaaggg tggcaagaag gccgcggcca
     6661 agaagtaaaa aatactacct ttaacgaaag taactgcttg atgaacatcc accaccatgg
     6721 agaaaataaa agaaagtgtt atttttagga aaaacacaag cacactgcca gctttctttt
     6781 ggtttaacgg ttttatttgc tctcaagaaa caaaatgatc ttaacagtcc aatctttaaa
     6841 gcacagtcta ccaaatagct aacgaccaga ggaccccgct caagtggggc acttgccgta
     6901 gatggtcttc cagttgttgg gatggttgta gcccagcagg atgggcaagt gcaggaacag
     6961 cgtgatatgt tcgccttgta tcgacgtgga caccttgacc cacttccgca gccactcgat
     7021 catggcctcg ctgctcaggc tgtcgggatt caatccgcgc agatagcagg agtagcgcaa
     7081 agcatccggt gtcaggttgt gaacgccacc ctcgcgggtt atggcctggt ccatgtagtg
     7141 caccaggaag gcgtgctcgt ggagccgatg tcgcttgaag atgctactgg gcagtccatg
     7201 aaggttgact aggtttctct ggaacgtatg cattagtgat atgctttagg ctgcataaaa
     7261 tgtgtcagat taaacgtaca acgtgcttcc gcgacattcc cagcagggaa tatggtcctt
     7321 cggcgaatat gtctttgaca tcgatgagca tctcaggcgt ggggtgtgtg ccgctgccca
     7381 gctggcccag gatgtcagca aaagccgagt gcttgggatg cgatgccgtc gctttgagct
     7441 tgtcctgcag gcagcgaaag acgtccttgt tattgcataa tcgccgcttc atgtagtagc
     7501 tctggaactc ggagcgctgc tgcggagtcc agaaatgggc agtgagaaag gagcgggggt
     7561 aataaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
     7621 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     7681 ggagactcta gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
     7741 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
     7801 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
     7861 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
     7921 caagaagaga actctgaata gggaattggg aattcgttaa cagatctgcg gccgcggctc
     7981 gagggtacct ctagatctgt tttggtcaat gacgtcgaca atggcctggt acctgccttg
     8041 gcaatgcgac cagtttgtac gaatctctcg aaagggaatt ccctttcgag agattcgtac
     8101 aaactggtcg cattgccaag gcaggtacca ggccattgtc gacgtcattg accaaaacag
     8161 atagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     8221 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     8281 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     8341 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     8401 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     8461 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     8521 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     8581 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     8641 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8701 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     8761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccactag tgtcgacgat
     8881 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     8941 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
     9001 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
     9061 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
     9121 cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca gcttttgttc
     9181 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9241 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9301 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9361 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10021 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    10081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11341 tccgcgcaca tttccccgaa aagtgccac
//
