LOCUS       sequence-82873-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag SKA3 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates JR 
            3rd, Cheeseman IM
  TITLE     The human kinetochore Ska1 complex facilitates microtubule 
            depolymerization-coupled motility.
  JOURNAL   Dev Cell. 2009 Mar;16(3):374-85. doi: 10.1016/j.devcel.2009.01.011.
  PUBMED    19289083
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.01"; journalName: "Dev Cell"; date: 
            "2009-03"; volume: "16"; issue: "3"; pages: "374-85"
COMMENT     Sequence Label: pIC306
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(3..213)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9..144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(14..311)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     309..329
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
       61 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
      121 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
      181 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
      241 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      301 ctttccacac cctaactgac acacattcca cagggtcgac cactgtgctg gcgaattgca
      361 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      421 tctacaaatg tggtatggct gattatgatc agttatctag atccggtgga tcccgggccc
      481 gcggtaccgt cgactgcaga attctcagtt ttctttgttg ctgacatctc ggatgttctg
      541 tccatgttta aggaaccttt tactgggtgg cactgcttta attgctattg gagtagctag
      601 gtttgagttg tattttgata aaagctggag aatatcttct ggaattttag ttacttctgg
      661 aggtgtaggt gttctgagca gattctcata agaagaaatc gtaggtgaag agggatctgt
      721 taaattctca aagcatgtgt ctgaatttaa aaccaacgaa gtacgatctt caacttccaa
      781 atcatttgat gaactatttg tttttgataa tgggtaattt gtggatacca aagctatgct
      841 gttctttgta gatggaattt tcaaaccagg agtacagaat gtaggtacca aa
//
