LOCUS       sequence-82874-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag SKA1 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates JR 
            3rd, Cheeseman IM
  TITLE     The human kinetochore Ska1 complex facilitates microtubule 
            depolymerization-coupled motility.
  JOURNAL   Dev Cell. 2009 Mar;16(3):374-85. doi: 10.1016/j.devcel.2009.01.011.
  PUBMED    19289083
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.01"; journalName: "Dev Cell"; date: 
            "2009-03"; volume: "16"; issue: "3"; pages: "374-85"
COMMENT     Sequence Label: pIC291
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(3..213)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9..144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(14..311)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     309..329
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
       61 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
      121 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
      181 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
      241 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      301 ctttccacac cctaactgac acacattcca cagggtcgac cactgtgctg gcgaattgca
      361 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      421 tctacaaatg tggtatggct gattatgatc agttatctag atccggtgga tcccgggccc
      481 gcggtcaggt tataacataa cgagtaagtc ctccccctcg gacctctgat agcctccggc
      541 agtgtcgtaa aatattcagt aacacgtgaa acttcttgtc agctttcaaa gttgtgaact
      601 cctttatgtc agcttccact ataaaataac gacctttggt atccttcgtt tcttcatcaa
      661 taaatctgtg atagagattt ctggtcacag aattcataga cttttttggc tgatgtagga
      721 ttttatattt actaattact gccttgttga tttctttaat aacatcatta atttgattat
      781 aggttaagcg gg
//
