LOCUS       sequence-82875-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Skinner PJ, Koshy BT, Cummings CJ, Klement IA, Helin K, Servadio A, 
            Zoghbi HY, Orr HT
  TITLE     Ataxin-1 with an expanded glutamine tract alters nuclear 
            matrix-associated structures.
  JOURNAL   Nature. 1997 Oct 30;389(6654):971-4.
  PUBMED    9353120
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-10-30"; volume: "389(6654)"; pages: "971-4"
COMMENT     Sequence Label: pcDNA1 Flag ATXN1[85Q]
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactat taacggccgc cagtgtgctg gaattccgtg
      121 actttccgtt tatcagacta aaatcagagc catccagaca gtgaaacagt caccgtggag
      181 gggggacggc gaaaaatgga ctacaaagac gacgacgaca aaaaatccaa ccaagagcgg
      241 agcaacgaat gcctgcctcc caagaagcgc gagatccccg ccaccagccg gtcctccgag
      301 gagaaggccc ctaccctgcc cagcgacaac caccgggtgg agggcacagc atggctcccg
      361 ggcaaccctg gtggccgggg ccacgggggc gggaggcatg ggccggcagg gacctcggtg
      421 gagcttggtt tacaacaggg aataggttta cacaaagcat tgtccacagg gctggactac
      481 tccccgccca gcgctcccag gtctgtcccc gtggccacca cgctgcctgc cgcgtacgcc
      541 accccgcagc cagggacccc ggtgtccccc gtacagtacg ctcacctgcc gcacaccttc
      601 cagttcattg ggtcctccca atacagtgga acctatgcca gcttcatccc atcacagctg
      661 atccccccaa ccgccaaccc cgtcaccagt gcagtggcct cggccgcagg ggccaccact
      721 ccatcccagc gctcccagct ggaggcctat tccactctgc tggccaacat gggcagtctg
      781 agccagacgc cgggacacaa ggctgagcag cagcagcagc agcagcagca
//
