LOCUS       sequence-82882-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone plasmid is pfastBacHTa from invitrogen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, 
            Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green 
            CB, Takahashi JS
  TITLE     Competing E3 ubiquitin ligases govern circadian periodicity by 
            degradation of CRY in nucleus and cytoplasm.
  JOURNAL   Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
  PUBMED    23452855
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1091-105"
COMMENT     Sequence Label: pfast bac cry1myc
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..129
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agctcggtac ccggggatcc tctagtcagc tgacgcgtta gtgaaccgtc agaattaacc
       61 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
      121 gatgacaagc ttgcggccgc gaattcatcg atagatctga tatcggtacc gatgaagagg
      181 aataatttct ctgccgtgaa taaggttgtc cagtcgtcac cagtagtgaa acagcccaaa
      241 cgtgggctct gctcttccct ccgccagacc catgcgctct ctgtccttct ggactggggg
      301 actttacctc accatgtcat attacagatc tttcagtacc ttcctttaat agatcgagcc
      361 cgggcttcgt ccgtgtgcag gagatggaat gaagtattcc acatccctga tctttggagg
      421 aagtttgagt ttgagctgaa ccaatcagca acttcttatt tcaagtccac tcatcccgac
      481 ctcattcaac agatcatcaa aaagcacgct gcacatcttc agtatgtcag ctttaaggtt
      541 gatagcagta ctgagtcggc agaagccgcc tgtgatatcc tttctcagct ggtaaattgt
      601 tctatccaga ccttgggatt gatttcaaca gccaaaccaa gtttcatgaa tgtgcccaag
      661 tctcactttg tgtcggcact tacggtcgtg tttgtcaact caaaatcgtt atcatcgatt
      721 aaaattgagg acacgccagt ggatgaccct tccctgaaga ttcttgtggc taacaacagt
      781 gacacccttc gactcctcaa aatgagtagc tgtcctcacg tttcatctga tgggatcctc
//
