LOCUS       sequence-82888-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone plasmid is pfastBacHTa from invitrogen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, 
            Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green 
            CB, Takahashi JS
  TITLE     Competing E3 ubiquitin ligases govern circadian periodicity by 
            degradation of CRY in nucleus and cytoplasm.
  JOURNAL   Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
  PUBMED    23452855
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1091-105"
COMMENT     Sequence Label: pfast bac Rbx-HA
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             206..232
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(601..619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(602..619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        673..691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             711..737
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
       61 ccaccatcgg gcgcggatcc cggtccgaag cgcgcggaat tcaaaggcct acgtcgacga
      121 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
      181 tatagggaga cccaagctta ccatgtaccc ttacgatgta ccggattacg caggatctat
      241 ggcggcagcg atggatgtgg ataccccgag cggcaccaac agcggcgcgg gcaagaagcg
      301 ctttgaagtg aaaaagtgga atgcagtagc cctctgggcc tgggatattg tggttgataa
      361 ctgtgccatc tgcaggaacc acattatgga tctttgcata gaatgtcaag ctaaccaggc
      421 gtccgctact tcagaagagt gtactgtcgc atggggagtc tgtaaccatg cttttcactt
      481 ccactgcatc tctcgctggc tcaaaacacg acaggtgtgt ccattggaca acagagagtg
      541 ggaattccaa aagtatgggc actaggaaaa gacctcgagc atgcatctag agggccctat
      601 tctatagtgt cacctaaatg ctagagctct ctggctaact agagaaccca ctgcttactg
      661 gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccatg tacccttacg
      721 atgtaccgga ttacgcagga tctatggcgg cagcgatgga tgtggatacc ccgagcggca
      781 ccaacagcgg cgcgggcaag aagcgctttg aagtgaaaaa gtggaatgca gtagccctct
      841 gggcctggga tattgtggtt gataactgtg ccatctgcag gaaccacatt atggatcttt
      901 gcatagaatg tcaagctaac ca
//
