LOCUS       sequence-82892-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone plasmid is pfastBacHTa from invitrogen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Yoo SH, Mohawk JA, Siepka SM, Shan Y, Huh SK, Hong HK, Kornblum I, 
            Kumar V, Koike N, Xu M, Nussbaum J, Liu X, Chen Z, Chen ZJ, Green 
            CB, Takahashi JS
  TITLE     Competing E3 ubiquitin ligases govern circadian periodicity by 
            degradation of CRY in nucleus and cytoplasm.
  JOURNAL   Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.
  PUBMED    23452855
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1091-105"
COMMENT     Sequence Label: pfast bac-flag Fbxl3
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             183..248
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
       61 ccaccatcgg gcgcggatcc cggtccgaag cgcgcggaat tcaaaggcct acgtcgacga
      121 gctcggtacc cggggatcct ctagtcagct gacgcgttag tgaaccgtca gaattaacca
      181 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
      241 atgacaagct tgcggccgcg aattcatcga tagatctgat atcggtaccg atgaaacgag
      301 gaggaagaga tagtgaccag gactctgctg aggaaggcac tgccgagaag cccaagagac
      361 cgaggacgac acaggagcgc tcgcagcctt gtgactgggg taacctcctg caggacatcg
      421 tcctccatgt ttttaagtat ttgcctcttc ttgaccgggc tcatgcttcc caagtgtgcc
      481 ggaattggaa tcaggtattt cacatgcctg acttgtggcg atgttttgaa tttgaactga
      541 atcagccagc gacatcttac ttgaaagcca cacacccaga gctgatcaaa cagattatca
      601 aaaggcactc aaaccaccta cagtatgtca gcttcaaggt ggacagcagc aaagaatcag
      661 ctgaagctgc ttgtgacata ctgtcgcagc ttgtgaattg ctctttaaaa actctcggac
      721 ttatctcgac tgcccggcca agctttatgg atctaccaaa gtctcacttt atctctgcac
      781 tgacagttgt gtttgtaaac tccaagtccc tgtcctcact taagatagac gacaccccag
      841 tcgatgaccc atcccttaaa gtcctcgtgg ccaacaacag cgacacactc aagctgttga
      901 aaatgagcag ctgtcctcac gtctctccag caggcattct gtgtgtggct gaccagtgtc
      961 atggcttacg agaactggcc ctgaactacc atttgctaag tgatgagttg ttgctcgcac
     1021 tgtct
//
