LOCUS       sequence-82898-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Lim J, Crespo-Barreto J, Jafar-Nejad P, Bowman AB, Richman R, Hill 
            DE, Orr HT, Zoghbi HY
  TITLE     Opposing effects of polyglutamine expansion on native protein 
            complexes contribute to SCA1.
  JOURNAL   Nature. 2008 Apr 10. 452(7188):713-8.
  PUBMED    18337722
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Apr 10. 452(7188):713-8."
COMMENT     Sequence Label: pDEST27-GST-ATXN1 FL(30Q)-S776A
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(85..96)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgactcacta tagggagaga gctatgacgt cgcatgcacg cgatcaacca ctttgtacaa
       61 gaaagttggc ttgcctacat tagaccggcc ttcaatgcaa atcttaacct cctgaggaat
      121 tagagaaggc ttaggaagag tcaaaggtgg ttcgtcttct gacttctcca gtttgcggct
      181 ctctggcgcc gcccacctcc tcttcctcgt tgccgcgggc ttgctgggtt ctattttggt
      241 gaggaagggc gctgcaggca atcccatttt ctctggaaac ttcagttcgc cattctcaga
      301 gagcatctgg gcactcccct ggttgattcc gttttcctgc tcggcatacc tgtgtctgct
      361 gcccgccagg ccgtcggcct ttgagtgctt cagcaggacg ctggcgggat ccacgggctg
      421 gcccttttta acagagccgt tcttcaggtt cttgagggta agcgagatgc agacatcccc
      481 aactgagagt ttggaacacg gcaaatcaaa gagctggctg gttctctccg gacagcagga
      541 tgaccagccc tgtccaaaca caaaaaaagg atactctacc aaaacttcaa cgctgacctg
      601 ggctcggtgc tccccgacgg cgaactgtat cacggccacg cccgggctat ggctgtcttc
      661 aatcctctct acggtgctgg agtcgatctt caggtcgttg cttatctctg cactctggat
      721 gaaatcttct gtttttaagt cttccacctt ctttagctcc ccgttggcca actggatgat
      781 ggagcctttc atgaagtagg gaggcagcgt agggggagcc gccgccgggg aggccacgga
      841 ctgcaccaca ggcaggtgga tctgggcctg caccatggct gggtaggcgg cctgggtgac
      901 cagggcctca gggttgaagt tctcgctctt gggaa
//
