LOCUS       sequence-82905-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lim J, Crespo-Barreto J, Jafar-Nejad P, Bowman AB, Richman R, Hill 
            DE, Orr HT, Zoghbi HY
  TITLE     Opposing effects of polyglutamine expansion on native protein 
            complexes contribute to SCA1.
  JOURNAL   Nature. 2008 Apr 10. 452(7188):713-8.
  PUBMED    18337722
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Apr 10. 452(7188):713-8."
COMMENT     Sequence Label: pDEST27-GST-ATXN1 FL(2Q)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(92..103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctatatacga ctcactatag ggagagagct atgacgtcgc atgcacgcga tcaaccactt
       61 tgtacaagaa agttggcttg cctacattag accggccttc aatgcaaatc ttaacctcct
      121 gaggaattag agaaggctta ggaagagtca aaggtggttc gtcttctgac ttctccagtt
      181 tgcggctctc tggcgccgac cacctcctct tcctcgttgc cgcgggcttg ctgggttcta
      241 ttttggtgag gaagggcgct gcaggcaatc ccattttctc tggaaacttc agttcgccat
      301 tctcagagag catctgggca ctcccctggt tgattccgtt ttcctgctcg gcatacctgt
      361 gtctgctgcc cgccaggccg tcggcctttg agtgcttcag caggacgctg gcgggatcca
      421 cgggctggcc ctttttaaca gagccgttct tcaggttctt gagggtaagc gagatgcaga
      481 catccccaac tgagagtttg gaacacggca aatcaaagag ctggctggtt ctctccggac
      541 agcaggatga ccagccctgt ccaaacacaa aaaaaggata ctctaccaaa acttcaacgc
      601 tgacctgggc tcggtgctcc ccgacggcga actgtatcac ggccacgccc gggctatggc
      661 tgtcttcaat cctctctacg gtgctggagt cgatcttcag gtcgttgctt atctctgcac
      721 tctggatgaa atcttctgtt tttaagtctt ccaccttctt tagctccccg ttggccaact
      781 ggatgatgga gcctttcatg aagtagggag gcagcgta
//
