LOCUS       sequence-82906-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP tagged MeCP2-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY
  TITLE     An AT-hook domain in MeCP2 determines the clinical course of Rett 
            syndrome and related disorders.
  JOURNAL   Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.
  PUBMED    23452848
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "984-96"
COMMENT     Sequence Label: pDEST-hMeCP2-GFP
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..708,709..711,712..714))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(38..58)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     653..681
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    725..749
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa gaaggaccat
       61 gtggtcacgc ttttcgttgg gatctttcga aagggcagat tgtgtcgaca ggtaatggtt
      121 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
      181 ttgaacggat ccatcttcaa tgttgtggcg aattttgaag ttagctttga ttccattctt
      241 ttgtttgtct gccgtgatgt atacattgtg tgagttatag ttgtactcga gtttgtgtcc
      301 gagaatgttt ccatcttctt taaaatcaat accttttaac tcgatacgat taacaagggt
      361 atcaccttca aacttgactt cagcacgcgt cttgtagttc ccgtcatctt tgaaagatat
      421 agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gccgtttcat
      481 atgatccgga taacgggaaa agcattgaac accataagag aaagtagtga caagtgttgg
      541 ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagct ttccgtatgt
      601 agcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa
      661 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttgctag ccattctaga
      721 tcgaaccact ttgtacaaga aagttgggct aactctctcg gtcacgggcg tccggctgtc
      781 cacaggctcc tctctgtttg gccttggcat ggaggatgaa acaatgtctt tgcgctctcc
      841 ctcccctcgg tgtttgtact tttctgcggc cgtggcggcg gtggcaaccg cgggctgagt
      901 cttagctggc tccttggggc agccgtcgct ctccagtgag cctcctctgg gcatcttctc
      961 ctctttgcag acgctgctgc tcaagtcctg gggctcaggg
//
