LOCUS       sequence-82927-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Kss1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan 
            DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A,
            Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, 
            Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein
            MB, Snyder M, Turk BE
  TITLE     Deciphering protein kinase specificity through large-scale analysis 
            of yeast phosphorylation site motifs.
  JOURNAL   Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482.
  PUBMED    20159853
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2010-02-16"; volume: "3(109):ra12. doi"; pages: " 
            10.1126/scisignal.2000482"
COMMENT     Sequence Label: pET28-Kss1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacctattcc atggtcttca ttagttcatc gtaaagcatg tctttgagca tttctatggg
      121 cacttcttcc tcctcttccg gacgcattat cttgttatcc agtttccaaa attcatcatc
      181 caaattaagt ggaggatatt ccggctcatc acttgggtca tggtacattg ccaggtaagg
      241 gtgtcttaaa gcttctgctg cgcttattct tttgtcagga ttgaattgaa gcattttgtc
      301 tagtaaatct atcatatctg gattcagatc ggtctttgac cagacggtct cccatggcaa
      361 gggtggcctc ataggtaagt ttgctatata ctctttagcc ctcttggatt tgatctgatt
      421 aaagtcttcg aaagatggag ttcccaagac ttctagaatt agccataatt gatgatgata
      481 gtctctgcct gggaacaaag gcttcccgga gaccatttca gccaaaatgc atccgcatga
      541 ccatatatcc atcgcagttg tgtactcttg aaaagttagc attatctcgg gtgccctgta
      601 ccatcgcgtt gcgacgtact ccgtcatgaa tcctaccaat gtttctcttg aatcgctact
      661 gctagctaaa cacctcgcta gtccaaaatc gcagactttg agatcacaat tggaatttag
      721 taacaggttt gatggcttta tgtctctatg gataacttgt gcactgtgaa tagactttaa
      781 ggctctgagg atttggtatg taaagtattg aacatggtca tcacttaaag tggaaa
//
