LOCUS       sequence-82941-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression vector with far-red FP mCardinal2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: pNCS-mCardinal2
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             44..66
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             107..139
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(143..151,152..166)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cttgacacct gatgcgatga atgatataat aggaaagtac tgttttgttt aactttaaga
       61 aggagatata catatgcggg gttctcatca tcatcatcat catggtatgg ctagcatgac
      121 tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggatc cgatggtgag
      181 caagggcgag gagctgatca aggagaacat gcccatgaag ctgtacatgg aaggcaccgt
      241 gaacaaccac cacttcaagt gcaccaccga aggggagggc aagccctacg agggcaccca
      301 gacccagagg attaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc
      361 cacctgcttt atgtacggga gcaagacctt catcaagcac cccaagggca tccccgattt
      421 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
      481 gggcgtgctt accgttaccc aggacaccag cctccaggac ggctgcttga tctacaacgt
      541 caagctcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
      601 ctgggaggcc accaccgaga ccctgtaccc cgctgacggc ggcctggaag gcagatgcga
      661 catggccctg aagctcgacg gcgggggcca cctgcactgc aacctgaaga ccacatacag
      721 atccaagaaa cccgctggca acctcaagat gcccggcgtc tactttgtgg accgcagact
      781 ggaaagaatc aaggaggccg acaatgagac ctacgtcgag cagcacgagg tggcc
//
