LOCUS       sequence-82944-e         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-G
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    154..278
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(303..308,309..326,327..333)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(453..472)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aatgaatgtt cgagccgagc agacgtgccg ctgccttcgt taatatcctt tgaataagcc
       61 aactttgaat cacaagacgc ataccaaacg aattcgcaga tctgcggccg cggctcgagg
      121 gccggatccg aattcccggg agagctcgat atcacaagtt tgtacaaaaa agctgaacga
      181 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      241 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
      301 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
      361 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      421 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      481 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
//
