LOCUS       sequence-82946-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Forebrain principal neuron expression of oChIEF kinetic variant 
            E163A/T199C with physically uncoupled mKate2 red fluorophore.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     ChIEF and oChIEF AAV vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..764)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattccctg caggggcgcg
       61 ccgtcgactc atctgtgccc cagtttgcta gggaggtcgc agtatctggc cacagccacc
      121 tcgtgctgct cgacgtaggt ctctttgtcg gcctccttga ttctttccag tcttctgtcc
      181 acatagtaga cgccgggcat cttgaggttc ttagcgggtt tcttggatct gtatgtggtc
      241 ttcaagttgc agatcaggtg gcccccgccc acgagcttca gggccatgtc ggctctgcct
      301 tccaggccgc cgtcagcggg gtacagggtc tcggtggagg cctcccagcc gagtgttttc
      361 ttctgcatca cagggccgtt ggatgggaag ttcacccctc tgatcttgac gttgtagatg
      421 aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat
      481 gtggtgactc tctcccatgt gaagccctcg gggaaggact gcttaaagaa gtcggggatg
      541 ccctgggtgt ggttgatgaa ggttttgctg ccgtacatga agctggtagc caggatgtcg
      601 aaggcgaagg ggagagggcc gccctcgacc gccttgattc tcatggtctg ggtgccctcg
      661 tagggcttgc cttcgccctc ggatgtgcac ttgaagtggt ggttgttcac ggtgccctcc
      721 atgtacagct tcatgtgcat gttctcctta atcagctcgc tcactggccc gggattctct
      781 tcgacatccc ctgcttgttt c
//
