LOCUS       sequence-82955-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath
            J
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and 
            cell death.
  JOURNAL   Cell. 2010 Aug 20. 142(4):590-600.
  PUBMED    20723759
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Aug 20. 142(4):590-600."
COMMENT     Sequence Label: pBABEpuro WT ATG3
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     293..313
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(348..365)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(375..416)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
       61 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
      121 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
      181 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
      241 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
      301 tgacacacat tccacagggt cgaccactgt gctggcgaat tccgtcaatg gtgatggtga
      361 tgatgaccgg tacgcgtaga atcgagaccg aggagagggt tagggatagg cttaccttcg
      421 aaccgcgggc cctctagact cgagcggccg caaatcattg tgaagtgtct tgtgtagtca
      481 tattctattg ttggaatgac agcttgaaca aatttcaaaa aaattaaaag atacatatga
      541 acaccaagct ctcccccgcc ttctgcaact gtctcaataa ttttcttcat cacttcagca
      601 tgcctgcatg ggtgaactga acacatagga ggtggtggga gatgaggatg gttttcaatg
      661 gtcactgttt tcttcacatg atcttgactg atgtcttcat acatgtgctc aactgttaaa
      721 ggctgccgtt gctcatcata gccaaacaac catagccgtg gtgtctggta atatttgtcg
      781 taagtgatgt acagatcgta tgttctcgtt tgtaaaatag catcttcacc tccagcgtca
      841 gccttagctt tgcaggcttc cactattttc cttgtgtcta gggtagcctc atctgttt
//
