LOCUS       sequence-82956-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath
            J
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and 
            cell death.
  JOURNAL   Cell. 2010 Aug 20. 142(4):590-600.
  PUBMED    20723759
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Aug 20. 142(4):590-600."
COMMENT     Sequence Label: pBABEpuro KR ATG3
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     292..312
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(347..364)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(374..415)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
       61 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      121 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
      181 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
      241 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
      301 gacacacatt ccacagggtc gaccactgtg ctggcgaatt ccgtcaatgg tgatggtgat
      361 gatgaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
      421 accgcgggcc ctctagactc gagcggccgc aaatcattgt gaagtgtctt gtgtagtcat
      481 attctattgt tggaatgaca gcttgaacaa atttcaaaaa aattaaaaga tacatatgaa
      541 caccaagctc tcccccgcct tctgcaactg tctcaataat tttcttcatc acttcagcat
      601 gcctgcatgg gtgaactgaa cacataggag gtggtgggag atgaggatgg ttttcaatgg
      661 tcactgttct cttcacatga tcttgactga tgtcttcata catgtgctca actgttaaag
      721 gctgccgttg ctcatcatag ccaaacaacc atagccgtgg tgtctggtaa tatttgtcgt
      781 aagtgatgta cagatcgtat gttctcgttt gtaaaatagc atcttcacct ccagcgtcag
      841 ccttagcttt gcaggcttcc actattttcc ttgtgtctag ggtagcctca tctgtttcca
      901 acaatccact ctcttcatat tc
//
