LOCUS       sequence-82958-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre dependent expression of oChIEF kinetic variant E163A/T199C with 
            physically uncoupled dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     ChIEF and oChIEF AAV vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc ggcgccacca
      181 tggtgagcag aagaccctgg ctgctggccc tggccctggc cgtggccctg gccgccggca
      241 gcgccggcgc cagcaccggc agcgacgcca ccgtgcccgt ggccacccag gacggccccg
      301 actacgtgtt ccacagagcc cacgagagaa tgctgttcca gaccagctac accctggaga
      361 acaacggcag cgtgatctgc atccccaaca acggccagtg cttctgcctg gcctggctga
      421 agagtaacgg caccaacgcc gagaagctgg ccgccaacat cctgcagtgg atcaccttcg
      481 ccctgagcgc cctgtgcctg atgttctacg gctaccagac ctggaagagt acctgcggct
      541 gggaggagat ctacgtggcc accatcgaga tgatcaagtt catcatagag tacttccacg
      601 agttcgacga gcccgccgtg atctacagca gcaacggcaa caagaccgtg tggctgagat
      661 acgccgcctg gctgctgacc tgccccgtgg tcctgatcca cctgagcaac ctgaccggcc
      721 tggccaacga ctacaacaag agaaccatgg gcctgctggt gagcgacatc ggctgtatcg
      781 tgtggggcac caccgccgcc ctgagcaagg gctacgtgag agtgatcttc ttcctgatgg
      841 gcctgtgcta cggcatctac accttcttca acgccgccaa ggtgtacatc gaggcctacc
      901 acaccgtg
//
