LOCUS       sequence-8296-ex        7029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Luong TT, Lee CY
  TITLE     Improved single-copy integration vectors for Staphylococcus aureus.
  JOURNAL   J Microbiol Methods. 2007 Jul . 70(1):186-90.
  PUBMED    17512993
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2007 Jul . 70(1):186-90."
COMMENT     Sequence Label: pLL29
FEATURES             Location/Qualifiers
     source          1..7029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(3285..4076)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(5328..6278)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      6326..6548
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttttt
       61 tattacctac aacttcttta gcacttaaac gaccatcatc agttattgga acaacgccat
      121 aatgcatatg tggtgttttt tcgtccatgt gaactgttgc atataataaa ttatctttac
      181 cgtattcttg ttctaaaaac tctttagcat attcaaaaaa ctgctttgta tcttctggcg
      241 tttgattatc aaagaaatca ttgtctgatg taattaaacc atcaatgtgt ttaatcgcgt
      301 ctgttctaat ttttcttttg cctgtataat tctgttcgat tttttcatca atcaagttat
      361 taaaattctg tttattagca tttaccaaat cataatttaa gtaagtttta ctatggtcta
      421 tatcttcatt ttcataatta ttattttctc tttgaacatg tttttgtatg cccgttgtat
      481 ttgttccaga tttaactttt gaaactctaa caatggaata agacatataa aaatcactcc
      541 taagcacaaa ggttttatgt gtatttaata gtttaagtct aacacactag acttattttt
      601 aataagtcgt taatacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa
      661 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa
      721 ctgcttttca gaacgtttaa atacaataat cgtcaaaaga caacataaaa taataaggat
      781 agccatggct acaagaatat tactatacac tccagaagaa taatttataa attccagaac
      841 tagtttacga ttaatcaatt gtagtgacaa taaacctcct acaattgcta tacctgttcc
      901 ctctgataaa aaacttgtga aatttagcaa actcattcca gaagcaactt cttcttcaga
      961 aagactactt gatactattt ttgatataac tgttttagta aaagataatc cgcccataac
     1021 aaatataaac ataaaagtag tcaaccacat actaaactca acaaaaaatg caatagttaa
     1081 aaaacttata gagatagaca atgatcctaa aataaaaaca aataatgatc cttttctatc
     1141 cactaaaaaa ccaccaaaat aaccaaaaac aataacactc atggttccag gaaaaataac
     1201 actattacct attgtcgcta catttacatg ataaatagtt ttcatcatat aaggcaccat
     1261 tgatataaaa ccagctacta tagaaaatat tagcccacca gaaaacaaac caagcataaa
     1321 cggaatgttt ttccctagtt taggattaat aaaagggtta gaaactcttg aaatatgttt
     1381 aataaaaatc acaaaaaaga ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt
     1441 aaataacata aaacatataa tacttataga cattaaaaca atacctacga tatctaatgt
     1501 attttttgtt gatttaccag gtaccattac tttaataaga aaaggtatag ttactattgt
     1561 aatcatagga agtataagta ggtaagacca atgaatataa tgtgctatta ttccccctat
     1621 tgaaggacct aacccttcac ctaaagctac aattgatcct ataaaaccaa aggctttgcc
     1681 ttgttttttt cttgtaatat ttctagctac aaccaccata atcagtgaag ggaatgcagc
     1741 agatcctact ccttgtacta acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat
     1801 aaaagcaatc aatgaaccaa gacagctcaa actaatacca ataattaaca atttttttat
     1861 atttatataa tcagataatt ttccatatac tgctgttcct atcgaaaaag ttaacatata
     1921 tgcagtgttt acccagtttg taattccagg agtagtatta aaatgatttg caatatcagg
     1981 taaagaaaca tttaaaacca tttcatttaa tacactaaaa aatgaaagaa tacaaagcca
     2041 aaataaaacg ctataaaaca aacctttaaa ttttttatat aaactaaaca attttacctc
     2101 tttacttgag gtgactaaag tttatagggg taaattattc tttcacctca tataaattcc
     2161 ccaaattttt aaatagacac ttcataaaaa aatcctccta aattagaata gtttaaatat
     2221 atcatttttg ttcagtaata ttaatatgta cactatttcc aaaatttaaa ttcatgttgc
     2281 caaaaatcga ttatgtcttt tgcggggcaa aaaaagggca gattatttga aaaagggcaa
     2341 acgcttgtgg aaaagctaaa aggttaaaaa tgacaaaaac cttgatacaa cagtgttttt
     2401 ggacgctcgt gtacgttaga gaatgaccgg tttaccatca tacaaggatg ggattaactt
     2461 gtgttaaaaa gcctttaata tcagttgtta caaaggattt gtagcgtctt taaaaataaa
     2521 aaagggcaga aaaagggcag atacctttta gtacacaagt ttttctaatt tttgctctaa
     2581 ctctctgtcc attttctctg ttacatgtgt atacaccttt atagtcgttt tttccagatc
     2641 tggaattaat gaggcattct aacattacag aaacaattca aacgtactct catttatata
     2701 ctgataaaaa acatcaagct atgagcatat ttgattaaac ggtatcaaat tggtatcaaa
     2761 taacaattaa ggagtttata aaatgcgtaa taacaagcct aaaataagta ttcaaaacga
     2821 cccatgggaa gtgaaattta tatacattta aatttcatga gacaataaac gttgatttaa
     2881 tgcgtttttt tgcctttttt attttcctta ttttttctgt tttacaacaa aatggtatca
     2941 aaaatggtat catttgtagt tattttagct tcacatatta aaacaaccac actcctaaat
     3001 taataggtgg tgtggttttg ttggttgtgt ggggatcgat agatctggat ctggatttta
     3061 atgcggatgt tgcgattact tcgccaacta ttgcgataac aagaaaaagc cagcctttca
     3121 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
     3181 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
     3241 agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc
     3301 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
     3361 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
     3421 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
     3481 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
     3541 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
     3601 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
     3661 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
     3721 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     3781 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     3841 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     3901 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     3961 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     4021 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg
     4081 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     4141 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
     4201 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     4261 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
     4321 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     4381 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
     4441 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
     4501 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     4561 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     4621 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa
     4681 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc
     4741 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagggg
     4801 aattaattcc cacgggtttt gctgcccgca aacgggctgt tctggtgttg ctagtttgtt
     4861 atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat ttcttccaga
     4921 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg
     4981 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa
     5041 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc
     5101 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt
     5161 tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat
     5221 ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt
     5281 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
     5341 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
     5401 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
     5461 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa
     5521 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
     5581 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
     5641 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
     5701 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
     5761 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
     5821 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
     5881 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
     5941 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
     6001 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
     6061 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg
     6121 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
     6181 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
     6241 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
     6301 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
     6361 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
     6421 agaccctctg taaattccgg tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
     6481 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
     6541 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
     6601 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
     6661 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
     6721 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
     6781 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
     6841 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
     6901 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
     6961 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
     7021 tactgtcgg
//
