LOCUS       sequence-82962-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(761..793)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(806..825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(807..825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(870..921)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(899..919)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc ctactccaag tagatatctt
       61 ctgtaggagt ggtatactcc acaaactgct tgtagaactg ctgaaatgca cgccccacag
      121 actccgccag ggctttggtg gaatcttcag acacaaagac atggcaggca aaccggtggt
      181 cagcagggtg cttagtgata aacccaaagt acttgttatt ctttggatgg tatccactga
      241 aagagatgtt ctttagctgg aagaagtggc tacatttatt tcccttggcc tccagagcat
      301 catcagcttt gacgcctatc ttgacacccc tgacactgat ctcaaggaca cagctggagg
      361 gcgggttaaa gtgcacggtg agccggcggg tggtggcgat cttttgcata gcagcacaga
      421 ggacatcatt gcccttgtga taaggaacct ggacagaccc caggaacttc acccggaact
      481 ggtcaatcca gtcgctgttt ttggcaaggg ctgccatgtg ctcaggctcc ttggtgacct
      541 caatggcata gtaggcaggg aagaccccgc gggctccggt gcgcatgtta taggcctcat
      601 accaatagtc ttctgcctgc agctccacca gcagggggtc atccacttcc agctcaagtt
      661 catcttcatg ccgaggcaca aacctgaata tagcccgatg ggtttgctca tgctcctccc
      721 cattgatgac gctggagaac agcccaaagg actcggatcc acccatttgc tgtccaccag
      781 tcatgctagc cataagcttg ggtctcccta tagtgagtcg tattaatttc gataagccag
      841 taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc ccaccgtaca
      901 cgcctaccgc ccatttgcgt caatggggc
//
