LOCUS       sequence-82965-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 
            17013874). For antisense cut with Spe1, transcribe with T7. For 
            sense cut with Not1, treat with T4 polymerase, transcribe with T3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Suriben R, Fisher DA, Cheyette BN
  TITLE     Dact1 presomitic mesoderm expression oscillates in phase with Axin2 
            in the somitogenesis clock of mice.
  JOURNAL   Dev Dyn. 2006 Nov;235(11):3177-83.
  PUBMED    17013874
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2006-11"; volume: "235(11)"; pages: "3177-83"
COMMENT     Sequence Label: pCR4-Dact1(1250-1601)
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(423..441)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(462..478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..478)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(475..497)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    486..502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(510..516,517..534,535..540))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    555..576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(693..710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agggcgaatt gaatttagcg gccgcgaatt cgcccttgcc tggccgcggg ccttcaaggt
       61 ggcgctcctt gcgacacccg ggttcctgtg tgcacggtga ggcctggcgg ccgcctgctg
      121 ccccggaatg aactgagctt tcaccagatg cccttcctcg aggctttgag atgagccctc
      181 gcttttgaag tccaaggccg gcctgtccag cgcgggcaca gcctggaggc tgttcttctg
      241 cgacatcttt ttcgggagct gcagggcttt gctctctgcg ggggcgacag ggcctctgcc
      301 aggacttgtt ttaggagaaa cagcggaggc ctgattgctt tccttcatgg attcccccag
      361 cccggcttga caccttgtga gctcttgaaa gggcgaattc gtttaaacct gcaggactag
      421 tccctttagt gagggttaat tctgagcttg gcgtaatcat ggtcatagct gtttcctgtg
      481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      721 gttcggctgc ggcgagcggt atcagctcac tcaaag
//
