LOCUS       sequence-82968-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre dependent expression of ChIEF kinetic variant E162A/T198C with 
            physically uncoupled dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     ChIEF and oChIEF AAV vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag ccaccatgtc
      181 gcggaggcca tggcttcttg ccctagcgct ggcagtggcg ctggcggccg gcagcgcagg
      241 agcctcgact ggcagtgacg cgacggtgcc ggtcgcgact caggatggcc ccgactacgt
      301 tttccaccgt gcccacgagc gcatgctctt ccaaacctca tacactcttg agaacaatgg
      361 ttctgttatt tgcatcccga acaacggcca gtgcttctgc ttggcttggc ttaaatccaa
      421 cggaacaaat gccgagaagt tggctgccaa cattctgcag tggattactt ttgcgctttc
      481 agcgctctgc ctgatgttct acggctacca gacctggaag tctacttgcg gctgggagga
      541 gatttacgtg gccacgatcg agatgatcaa gttcatcatc gagtatttcc atgagtttga
      601 cgaacctgcg gtgatctact catccaacgg caacaagacc gtgtggcttc gttacgcggc
      661 ctggctgctg acctgccctg tcgttcttat ccatctgagc aaccttacgg gtctggcgaa
      721 cgactataac aagcgtacca tgggtctgct ggtgtcagat atcggctgta tcgtgtgggg
      781 caccacgg
//
