LOCUS       sequence-82980-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces
            ~10-fold higher titer virus. 3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiCRISPR v2
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttaacttgc tatttctagc tctaaaacag agacgtacaa aaaagagcaa gaagctaaaa
       61 aagatttaaa aattattttt agcgcagtta atggaacagg aactaaattt accccaaaaa
      121 tattacgtga atcaggatat aacgttattg aggttgaaga gcatgcattt gaagatgaaa
      181 catttaaaaa tgttgtaaat ccaaatccag aatttgatcc tgcatgaaaa ataccgcttg
      241 aatatggtat taaacatgat gcagatatta ttattatgaa tgacccagat gctgacagat
      301 ttggaatggc aataaaacat gatggtcatt ttgtaagatt agatggaaat caaacaggac
      361 caattttaat tgattgaaaa ttatcaaatc taaaacgctt aaatagcatt ccaaaaaatc
      421 cggctctata ttcaagtttt gtaacaagtg atttgggtga tagaatcgct catgaaaaat
      481 atggagttaa tattgtaaaa actttaactg gatttaaatg aatgggtaga gaaattgcta
      541 aagaagaaga taacggatta aattttgttt ttgcttatga agaaagttat ggatatgtaa
      601 ttgatgactc agctagagat aaagatggaa tacaagcttc tatattaata gcagaggctg
      661 cttgatttta taaaa
//
