LOCUS       sequence-82984-e        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal His tag fused human codon-optimized Cas9 
            nuclease having C-terminal Cysteine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H
  TITLE     Gene disruption by cell-penetrating peptide-mediated delivery of 
            Cas9 protein and guide RNA.
  JOURNAL   Genome Res. 2014 Apr 2.
  PUBMED    24696462
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Apr 2."
COMMENT     Sequence Label: pET28a/Cas9-Cys
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agactttccc gttacccgag atcaccaagg cccccctgag cgccagcatg atcaagcgct
       61 acgacgagca ccaccaggac ctgaccctgc tgaaggccct ggtgcgccag cagctgcccg
      121 agaagtacaa ggagatcttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgacg
      181 gcggcgccag ccaggaggag ttctacaagt tcatcaagcc catcctggag aagatggacg
      241 gcaccgagga gctgctggtg aagctgaacc gcgaggacct gctgcgcaag cagcgcacct
      301 tcgacaacgg cagcatcccc caccagatcc acctgggcga gctgcacgcc atcctgcgcc
      361 gccaggagga cttctacccc ttcctgaagg acaaccgcga gaagatcgag aagatcctga
      421 ccttccgcat cccctactac gtgggccccc tggcccgcgg caacagccgc ttcgcctgga
      481 tgacccgcaa gagcgaggag accatcaccc cctggaactt cgaggaggtg gtggacaagg
      541 gcgccagcgc ccagagcttc atcgagcgca tgaccaactt cgacaagaac ctgcccaacg
      601 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
      661 ccaaggtgaa gtacgtgacc gagggcatgc gcaagcccgc cttcctgagc ggcgagcaga
      721 agaaggccat cgtggacctg ctgttcaaga ccaaccgcaa ggtgaccgtg aagcagctga
      781 aggaggacta cttcaagaag atcgagtgct tcgacagcgt ggagatcagc ggcgtggagg
      841 accgcttcaa cgccagcctg ggcacctacc acgacctgct gaagatcatc aaggacaagg
      901 acttcctgga caacgaggag aacgaggaca tcctggagga catcgtgctg accctgaccc
      961 tgttcgagga ccgcgagatg atcgaggagc gcctgaagac ct
//
