LOCUS       sequence-82985-e        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal His tag fused human codon-optimized Cas9 
            nuclease having C-terminal Cysteine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H
  TITLE     Gene disruption by cell-penetrating peptide-mediated delivery of 
            Cas9 protein and guide RNA.
  JOURNAL   Genome Res. 2014 Apr 2.
  PUBMED    24696462
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Apr 2."
COMMENT     Sequence Label: pET28a/Cas9-Cys
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             978..998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             999..1025
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             999..1025
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1047..1064
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagaccgaga tcaccctggc caacggcgag atcgcaagcg cccctgatcg agaccaacgc
       61 gagaccgcga gatcgtgtgg gacaagagcc gcgacttcgc caccgtgcgc aaggtgctga
      121 gcatgcccca ggtgaacatc gtgaagaaga ccgaggtgca gaccggcggc ttcagcaagg
      181 agagcatcct gcccaagcgc aacagcgaca agctgatcgc ccgcaagaag gactgggacc
      241 ccaagaagta cggcggcttc gacagcccca ccgtggccta cagcgtgctg gtggtggcca
      301 aggtggagaa gggcaagagc aagaagctga agagcgtgaa ggagctgctg ggcatcacca
      361 tcatggagcg cagcagcttc gagaagaacc ccatcgactt cctggaggcc aagggctaca
      421 aggaggtgaa gaaggacctg atcatcaagc tgcccaagta cagcctgttc gagctggaga
      481 acggccgcaa gcgcatgctg gccagcgccg gcgagctgca gaagggcaac gagctggccc
      541 tgcccagcaa gtacgtgaac ttcctgtacc tggccagcca ctacgagaag ctgaagggca
      601 gccccgagga caacgagcag aagcagctgt tcgtggagca gcacaagcac tacctggacg
      661 agatcatcga gcagatcagc gagttcagca agcgcgtgat cctggccgac gccaacctgg
      721 acaaggtgct gagcgcctac aacaagcacc gcgacaagcc catccgcgag caggccgaga
      781 acatcatcca cctgttcacc ctgaccaacc tgggcgcccc cgccgccttc aagtacttcg
      841 acaccaccat cgaccgcaag cgctacacca gcaccaagga ggtgctggac gccaccctga
      901 tccaccagag catcaccggt ctgtacgaga cccgcatcga cctgagccag ctgggcggcg
      961 acggcggctc cggacctcca aagaaaaaga gaaaagtata cccctacgac gtgcccgact
     1021 acgcctgtta agcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
     1081 acaaagcccg aaaagaaaag ctgaagtttt gt
//
