LOCUS       sequence-82986-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal His tag fused human codon-optimized Cas9 
            nuclease having C-terminal Cysteine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H
  TITLE     Gene disruption by cell-penetrating peptide-mediated delivery of 
            Cas9 protein and guide RNA.
  JOURNAL   Genome Res. 2014 Apr 2.
  PUBMED    24696462
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Apr 2."
COMMENT     Sequence Label: pET28a/Cas9-Cys
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accttcaagg aggacatcca gaaggcccag gtgagcggca gggcgacagc tgcacgagca
       61 catcgccaac ctggccggca gcccgccatc agaagggcat ctgcagaccg tgaaggtggt
      121 ggacgagctg gtgaaggtga tgggccgcca caagcccgag aacatcgtga tcgagatggc
      181 ccgcgagaac cagaccaccc agaagggcca gaagaacagc cgcgagcgca tgaagcgcat
      241 cgaggagggc atcaaggagc tgggcagcca gatcctgaag gagcaccccg tggagaacac
      301 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aacggccgcg acatgtacgt
      361 ggaccaggag ctggacatca accgcctgag cgactacgac gtggaccaca tcgtgcccca
      421 gagcttcctg aaggacgaca gcatcgacaa caaggtgctg acccgcagcg acaagaaccg
      481 cggcaagagc gacaacgtgc ccagcgagga ggtggtgaag aagatgaaga actactggcg
      541 ccagctgctg aacgccaagc tgatcaccca gcgcaagttc gacaacctga ccaaggccga
      601 gcgcggcggc ctgagcgagc tggacaaggc cggcttcatc aagcgccagc tggtggagac
      661 ccgccagatc accaagcacg tggcccagat cctggacagc cgcatgaaca ccaagtacga
      721 cgagaacgac aagctgatcc gcgaggtgaa ggtgatcacc ctgaagagca agctggtgag
      781 cgacttccgc aaggacttcc agttctacaa ggtgcgcgag atcaacaact accaccacgc
      841 ccacgacgcc tacctgaacg ccgtggtggg caccgccctg atcaagaagt accccaagct
      901 ggagagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcgcaaga tgatcgccaa
      961 gagcgagcag gagatcggca aggccaccgc caagtacttc ttctacagca acatcatgaa
     1021 cttcttcaag accgagatca ccctggccaa cggcgagatc cgcaagcgcc ccctatcaac
     1081 cacgatcgtt ctccgcaaaa aact
//
