LOCUS       sequence-82992-e        9268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea 
            agglomerans) Eho10, and produces beta-carotene in E. coli when 
            complemented with a gene encoding geranylgeranyl diphosphate 
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A portfolio of plasmids for identification and analysis of 
            carotenoid pathway enzymes: Adonis aestivalis as a case study.
  JOURNAL   Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
  PUBMED    17634749
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photosynth 
            Res."; date: "2007-05"; volume: "92(2)"; pages: "245-59. Epub 2007 
            Jul 17"
COMMENT     Sequence Label: pAC-94N
FEATURES             Location/Qualifiers
     source          1..9268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6956..6975)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(8828..9268,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgttct cgacccccgt cgcgccgtaa ctcaggctag cgcaggtttt gagcgctggc
     1741 gctttaccca ctgcgccctg ccagagctga attttagcga catcacgctg gaaaccacct
     1801 tcctgaatcg gcagctacag gctccgctgc tgatcagctc catgaccggc ggcgttgagc
     1861 gctcgcgcca tatcaaccgc cacctcgccg aggcggcgca ggtgctaaaa attgcgatgg
     1921 gggtgggctc ccagcgcgtc gccattgaga gcgacgcggg cttagggctg gataaaaccc
     1981 tgcggcagct ggctccggac gtgccgctgc tggcgaacct cggcgcggcg cagctgaccg
     2041 gcagaaaagg tattgattac gcccgacggg ccgtggagat gatcgaggcg gatgcgctga
     2101 ttgtgcacct aaacccgctg caggaggcgc tacagcccgg cggcgatcgc gactggcgcg
     2161 gacggctggc ggctattgaa actctggtcc gcgagctgcc cgttccgctg gtggtgaaag
     2221 aggtgggagc cggtatctcc cgaaccgtgg ccgggcagct gatcgatgcc ggcgttaccg
     2281 tgattgacgt cgcgggcgcg ggcggcacca gctgggccgc cgttgaaggc gagcgggcgg
     2341 ccaccgagca gcagcgcagc gtggccaacg tctttgccga ctgggggatc cccaccgctg
     2401 aggcgctggt tgacattgcc gaggcctggc cgcagatgcc ccttattgcc tcgggcggga
     2461 ttaaaaacgg cgtcgaccag ccgcgctacg ttgccgaggc taatctggtg atcacccacg
     2521 gcggtctcaa taccgtactg gatgcgctgg ctgccgcgac gccggtgctg gcggtgccac
     2581 tctctttcga ccagcccgcc gtggctgccc ggctggtcta taacgggctg ggtcgccggg
     2641 tatcgcgctt tgccagacag cagacgctgg cggatgagat tgcccaactg ctgggggatg
     2701 agacgctgca tcagcgtctg gcgacggccc gccagcagct taacgacgcc gggggcacgc
     2761 cccgtgcggc gaccctgatt gaacaggcca tagcagggag tgagagcgta tcgtgaggga
     2821 tctgatttta gtcggcggcg gcctggccaa cgggctgatc gcctggcgtc tgcgccagcg
     2881 ctacccgcag cttaacctgc tgctgatcga ggccggggag cagcccggcg ggaaccatac
     2941 ctggtcattc catgaagacg atctgactcc cgggcagcac gcctggctgg ccccgctggt
     3001 ggcccacgcc tggccgggct atgaggtgca gtttcccgat cttcgccgtc gcctcgcgcg
     3061 cggctactac tccattacct cagagcgctt tgccgaggcc ctgcatcagg cgctggggga
     3121 gaacatctgg ctaaactgtt cggtgagcga ggtgttaccc aatagcgtgc gccttgccaa
     3181 cggtgaggcg ctgcttgccg gagcggtgat tgacggacgc ggcgtgaccg ccagttcggc
     3241 gatgcaaacc ggctatcagc tctttcttgg tcagcagtgg cggctgacac agccccacgg
     3301 cctgaccgta ccgatcctga tggatgccac ggtggcgcag cagcagggct atcgctttgt
     3361 ctacacgctg ccgctctccg ccgacacgct gctgatcgag gatacgcgct acgccaatgt
     3421 cccgcagcgt gatgataatg ccctacgcca gacggttacc gactatgctc acagcaaagg
     3481 gtggcagctg gcccagcttg aacgcgagga gaccggctgt ctgccgatta ccctggcggg
     3541 tgacatccag gctctgtggg ccgatgcgcc gggcgtgccg cgctcgggaa tgcgggctgg
     3601 gctatttcac cctaccactg gctattcgct gccgctggcg gtggcccttg ccgacgcgat
     3661 tgccgacagc ccgcggctgg gcagcgttcc gctctatcag ctcacccggc agtttgccga
     3721 acgccactgg cgcaggcagg gattcttccg cctgctgaac cggatgcttt tcctggccgg
     3781 gcgcgaggag aaccgctggc gggtgatgca gcgcttttat gggctgccgg agcccaccgt
     3841 agagcgcttt tacgccggtc ggctctctct ctttgataag gcccgcattt tgacgggcaa
     3901 gccaccggtt ccgctgggcg aagcctggcg ggcggcgctg aaccattttc ctgacagacg
     3961 agataaagga tgaaaaaaac cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg
     4021 attcgcctgc aggcggcagg gatcccaacc gtactgctgg agcagcggga caagcccggc
     4081 ggtcgggcct acgtctggca tgaccagggc tttacctttg acgccgggcc gacggtgatc
     4141 accgatccta ccgcgcttga ggcgctgttc accctggccg gcaggcgcat ggaggattac
     4201 gtcaggctgc tgccggtaaa acccttctac cgactctgct gggagtccgg gaagaccctc
     4261 gactatgcta acgacagcgc cgagcttgag gcgcagatta cccagttcaa cccccgcgac
     4321 gtcgagggct accggcgctt tctggcttac tcccaggcgg tattccagga gggatatttg
     4381 cgcctcggca gcgtgccgtt cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg
     4441 cttaagctcc aggcgtggca gagcgtctac cagtcggttt cgcgctttat tgaggatgag
     4501 catctgcggc aggccttctc gttccactcc ctgctggtag gcggcaaccc cttcaccacc
     4561 tcgtccatct acaccctgat ccacgccctt gagcgggagt ggggggtctg gttccctgag
     4621 ggcggcaccg gggcgctggt gaacggcatg gtgaagctgt ttaccgatct gggcggggag
     4681 atcgaactca acgcccgggt cgaagagctg gtggtggccg ataaccgcgt aagccaggtc
     4741 cggctggcgg atggtcggat ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg
     4801 aacacctata aaaagctgct cggccaccat ccggtggggc agaagcgggc ggcagcgctg
     4861 gagcgcaaga gcatgagcaa ctcgctgttt gtgctctact tcggcctgaa ccagcctcat
     4921 tcccagctgg cgcaccatac catctgtttt ggtccccgct accgggagct gatcgacgag
     4981 atctttaccg gcagcgcgct ggcggatgac ttctcgctct acctgcactc gccctgcgtg
     5041 accgatccct cgctcgcgcc tcccggctgc gccagcttct acgtgctggc cccggtgccg
     5101 catcttggca acgcgccgct ggactgggcg caggaggggc cgaagctgcg cgaccgcatc
     5161 tttgactacc ttgaagagcg ctatatgccc ggcctgcgta gccagctggt gacccagcgg
     5221 atctttaccc cggcagactt ccacgacacg ctggatgcgc atctgggatc ggccttctcc
     5281 atcgagccgc tgctgaccca aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt
     5341 gccaacctct acctggtggg cgcaggtact caccctgggg cgggcattcc tggcgtagtg
     5401 gcctcggcga aagccaccgc cagcctgatg attgaggatc tgcaatgagc caaccgccgc
     5461 tgcttgacca cgccacgcag accatggcca acggctcgaa aagttttgcc accgctgcga
     5521 agctgttcga cccggccacc cgccgtagcg tgctgatgct ctacacctgg tgccgccact
     5581 gcgatgacgt cattgacgac cagacccacg gcttcgccag cgaggccgcg gcggaggagg
     5641 aggccaccca gcgcctggcc cggctgcgca cgctgaccct ggcggcgttt gaaggggccg
     5701 agatgcagga tccggccttc gctgcctttc aggaggtggc gctgacccac ggtattacgc
     5761 cccgcatggc gctcgatcac ctcgacggct ttgcgatgga cgtggctcag acccgctatg
     5821 tcacctttga ggatacgctg cgctactgct atcacgtggc gggcgtggtg ggtctgatga
     5881 tggccagggt gatgggcgtg cgggatgagc gggtgctgga tcgcgcctgc gatctggggc
     5941 tggccttcca gctgacgaat atcgcccggg atattattga cgatgcggct attgaccgct
     6001 gctatctgcc cgccgagtgg ctgcaggatg ccgggctgac cccggagaac tatgccgcgc
     6061 gggagaatcg ggccgcgctg gcgcgggtgg cggagcggct tattgatgcc gcagagccgt
     6121 actacatctc ctcccaggcc gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg
     6181 ccaccgcccg cagcgtctac cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg
     6241 cctgggatcg ccgccagcac accagcaaag gtgaaaaaat tgccatgctg atggcggcac
     6301 cggggcaggt tattcgggcg aagacgacga gggtgacgcc gcgtccggcc ggtctttggc
     6361 agcgtcccgt ttaggcgggc ggccatgacg ttcacgcagg atcgcctgta ggtcggcagg
     6421 cttgcgggcg taaataaaac cgaaggagac gcagccctcc cggccgcgca ccgcgtggtg
     6481 caggcggtgg gcgacgtaga gccgcttcag gtagccccgg cgcgggatcc cgcctcgccg
     6541 tgctgtccgg tctcaacctg atccgccaga atcgagccaa cgggatcggc cagcacgaat
     6601 tcggtatgcg gagagtgttt ggcaaaccat gcctgcaagc cacccagcgt gccgccggag
     6661 ccaacgccta ctaccacggc atcaaccctg cctgccagct ggtcgaacag ctccggtgcg
     6721 gtggtggtgg cgtgcgccag cgggttggcc gggttagaga actgatcaat atagtaagca
     6781 cccggcgtct cctctgccag gcggcgggcg tagtcctgat agtactccgg gtggcccttt
     6841 gtcacgtcgg agcgggtcag gcgcacatca acacccagcg cacgcaggtg gtagatcctc
     6901 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     6961 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     7021 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     7081 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     7141 ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca
     7201 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     7261 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     7321 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     7381 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     7441 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     7501 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     7561 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     7621 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat
     7681 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     7741 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
     7801 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
     7861 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
     7921 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
     7981 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
     8041 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
     8101 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
     8161 cccctacgtg ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga
     8221 tactatgact gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt
     8281 ccgcaccgct gtccggtagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     8341 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg cccaacagtc
     8401 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgccctgc accattatgt
     8461 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     8521 cgaagcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     8581 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     8641 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     8701 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     8761 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
     8821 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     8881 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     8941 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     9001 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     9061 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     9121 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     9181 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     9241 ccagctcacc gtctttcatt gccatacg
//
