LOCUS       sequence-83006-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null TD Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-TDcasN-
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(295..322)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gccttcagca gcttgtagtc ggtgtcggtc acggcccagc ccacgctgcc ggtgcccacg
       61 gccaggccca ggaagtagtc cttgatctcc ttcttcattt ttgcctcctc ctctagtaaa
      121 agttaaacaa aattatttgt agagggaaac cgttgtggtc tccctgaata tatcatacga
      181 gccttatgca tgcccgtaaa gttatccagc aaccactcat agacctaggg cagcagatag
      241 ggacgacgtg gtgttagctg tgctctagaa gctagcagag tttgtagaaa cgcaaaaagg
      301 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
      361 gcccgccacc ctccgggccg ttgcttcctg caggcacggc agcggctgcg cagggctctt
      421 ctcgatcaat atcgttcact catccaatct cactgacggt taggcgtgcc tggtaccctg
      481 ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat
      541 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
      601 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
      661 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
      721 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
      781 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatag
//
