LOCUS       sequence-83009-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split fluorescence protein to measure caspase-2 
            dimerization in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon 
            CP, Flanagan JM, Beere HM, Green DR
  TITLE     Characterization of cytoplasmic caspase-2 activation by induced 
            proximity.
  JOURNAL   Mol Cell. 2009 Sep 24;35(6):830-40. doi: 
            10.1016/j.molcel.2009.07.023.
  PUBMED    19782032
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.07"; journalName: "Mol Cell"; date: 
            "2009-09-24- 24"; volume: "35"; issue: "6"; pages: "830-40"
COMMENT     Sequence Label: pBiFC-flag-hCaspase 2 FL (C303S)-VN173
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat tgatctacca tggactacaa agacgatgac
       61 gacaagcttg cggccgctat ggccgctgac aggggacgca ggatattggg agtgtgtggc
      121 atgcatcctc atcatcagga aactctaaaa aagaaccgag tggtgctagc caaacagctg
      181 ttgttgagcg aattgttaga acatcttctg gagaaggaca tcatcacctt ggaaatgagg
      241 gagctcatcc aggccaaagt gggcagtttc agccagaatg tggaactcct caacttgctg
      301 cctaagaggg gtccccaagc ttttgatgcc ttctgtgaag cactgaggga gaccaagcaa
      361 ggccacctgg aggatatgtt gctcaccacc ctttctgggc ttcagcatgt actcccaccg
      421 ttgagctgtg actacgactt gagtctccct tttccggtgt gtgagtcctg tcccctttac
      481 aagaagctcc gcctgtcgac agatactgtg gaacactccc tagacaataa agatggtcct
      541 gtctgccttc aggtgaagcc ttgcactcct gaattttatc aaacacactt ccagctggca
      601 tataggttgc agtctcggcc tcgtggccta gcactggtgt tgagcaatgt gcacttcact
      661 ggagagaaag aactggaatt tcgctctgga ggggatgtgg accacagtac tctagtcacc
      721 ctcttcaagc ttttgggcta tgacgtccat gttctatgtg accagactgc acaggaaatg
      781 caagagaaac tgcagaattt tgcacagtta cctgcacacc gagtcacgga ctcctgcatc
      841 gtggcactcc tctcgcatgg tgtggagggc gccatctatg gtgtggatgg gaaactgctc
      901 cagctccaag aggtttttca gctctttgac aacgccaact gcccaagcct acagaacaaa
      961 ccaaaaatgt tcttcatcca ggcctcccgt ggagatgaga ctgatcgtgg ggttgaccaa
     1021 caagatggaa a
//
