LOCUS       sequence-83011-e         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dual shRNA expression cassette entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pENTR.hU6hH1
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        61..275
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     196..215
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(290..306)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(291..307)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(317..341)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctttatata tcttgtggaa aggacgaaac accggtccgc aggtatgcac gcgtgaattc
       61 gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa
      121 cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc
      181 tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg
      241 gatttgggaa tcttataagt tctgtatgag accacagatc taagcttatc gataccgtcg
      301 acctcgagat atctagaccc agctttcttg tacaaagttg gcattataa
//
