LOCUS       sequence-83012-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre/lox mammalian expression and gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pENTR.CMV.ON
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(41..74)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(171..204)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(270..287)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    276..500
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(529..628)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(740..759)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct ataacttcgt atagcataca
       61 ttatacgaag ttatagctcc taggaaattt aaataaggcg cgccaagtta acgggcggcc
      121 gcatggatcc gccctcgagc cgtttaaaca agaattcgtc gagggaccta ataacttcgt
      181 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
      241 cgatagctta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      301 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      361 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      421 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      481 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agatctagac ccagctttct
      541 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      601 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
      661 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
      721 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga a
//
