LOCUS       sequence-83014-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre.
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1984
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..109
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     507..527
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca
       61 gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc
      121 agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg
      181 atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc
      241 agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag
      301 tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa
      361 acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca
      421 gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat
      481 acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac
      541 cagtcaatct ttcacaaatt ttgtaatcca gaggttgagc gatcgcggcc gctcagtccc
      601 catcctcgag cagcctcacc atggccccag tctcagagtc caggtttctg atgtagttca
      661 tcactatgtt cac
//
