LOCUS       sequence-83016-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression and gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pENTR.CMV
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(179..196)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    185..409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(438..537)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(649..668)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(727..746)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagactc gaaattccac
      121 cacactggac tagtggatcc gagctcggta ccaagcttaa gtttaaaccg ctgatcagcc
      181 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      241 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
      301 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
      361 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
      421 gaaagaacca gatctagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
      481 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
      541 tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      601 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      661 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
      721 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
      781 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      841 atgatgtt
//
