LOCUS       sequence-83030-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces
            ~10-fold higher titer virus. 3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiCRISPR v2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    52..173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(63..82)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     117..136
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(303..677)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(696..743)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(859..878)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca
       61 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      121 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg
      181 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
      241 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg
      301 tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg
      361 aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg
      421 aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac
      481 acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc
      541 acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc
      601 cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca
      661 ctggtcaact tggccatggt ttagttcctc accttgtcgt attatactat gccgatatac
      721 tatgccgatg attaattgtc aacacgtgct gatcagatcc gaaaatggat atacaagctc
      781 ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
      841 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatgg
//
