LOCUS       sequence-83032-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for TAZ S89A mutant with N-terminal 
            FLAG and His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EF-FH-TAZ S89A-ires-blast
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..427)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(633..652)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 aaccggtacg cgggccgcca cgaagtgctt agccctccca cacataacca gagggcagca
       61 attcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc
      121 caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt
      181 tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc
      241 ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg acaccagtga
      301 agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca gagatgggga
      361 tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct
      421 tggccatggt ttagttcctc accttgctag aggatcccgg gttgtggcaa gcttatcatc
      481 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc
      541 tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg
      601 gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc
      661 tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat
      721 cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc
      781 tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccaga
//
