LOCUS       sequence-83033-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a myc-tagged version of hCas9 in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB14
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(522..542)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg gctgcatggc
       61 gcgcctttgc gatcgcagac ctaggagact tgtgacaaaa ttggatccga ctttattgat
      121 tacgttgtta agagaacaaa tcttttacaa ctgaattcat ttgttctcgt ttcatttttt
      181 ttcgcaaaac attgatcgag aattcgattg atttccgatt cgaatgctag gagacttgtg
      241 aacaaattgg atccgacttt attgattacg ttgttaagag aacaaatctt ttacaactga
      301 attcatttgt tctcgtttca ttttttttcg caaaacattg atcgagaatt cgattgattt
      361 ccgattcgaa tgctagctat acgcgttata ccggtatcct gcaggtagca tgcaagagat
      421 cttataggcc tcgagttaac gttcgaggtc gactctagat aaagctttag agctcttctt
      481 aagaaggatc cagatccact agtggcctat gcggccgctc acaccttcct cttcttcttg
      541 gggtcagccc tgctgtctcc accgagctga gagaggtcga ttcttgtttc atagagcccc
      601 gtaattgact gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt gtaccgcttt
      661 ctgtctatgg tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt ggtcagagta
      721 aacaagtgga taatgttttc tgcctgctcc ctgatgggct tatccctgtg cttattgtaa
      781 gcagaaagca ccttatcgag gttagcgtcg gcgaggatca ctcttttgga gaattcgctt
      841 atttgctcga tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag ctgcttctgc
      901 tcattatctt cgggagaccc tttgagcttt tcata
//
